Species : |
Human |
Source : |
Sf9 Cells |
Tag : |
His |
Description : |
β2 Glycoprotein I (β2 GP I) is a serum protein with a single polypeptide chain consisting of 326 amino acids and having a molecular weight of 50 kDa. The protein is also known by the alternative name Apolipoprotein H (ApoH) which gives an indication of its phospholipid binding function. Since the detection of β2 Glycoprotein I as a target of antiphospholipid antibodies in 1990 the discussion about the clinical relevance of β2 GP I is still controversial. It is well established that anticardiolipin antibodies are a very heterogeneous group and that β2 GP I is only one of several potential targets for anticardiolipin antibodies. Nevertheless it is generally accepted that the binding of β2 GP I to anionic lipids or surface is essential for the differential detection of anticardiolipin antibodies associated with autoimmune diseases (versus those associated with infectious diseases like syphilis, tuberculosis, AIDS, etc). |
Physical Appearance : |
Sterile Filtered clear solution (1mg/ml). |
Purity : |
Greater than 90% as determined by SDS-PAGE. |
Formulation : |
2GP-1 is supplied in 16mM HEPES buffer pH-7.2, 200mM NaCl, and 20% glycerol. |
Immunological Functions : |
1. Binds IgA & IgG-type human auto-antibodies, Auto-antibodies to β2 glycoprotein I recognize conformation dependent and linear epitopes. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including a reference calibrator). |
Applications : |
Western-Blot with monoclonal anti-hexa-His-tag antibody and Goodpasture patient sera. |
Coating Concentration : |
0.5-1 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |
Storage : |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |