Recombinant Human ATG5 Protein, His-tagged

• Cat.No.: ATG5-260H
• Product Overview: Recombinant Human ATG5 Protein (Met1-Asp275) with N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.1 kDa
  Accurate Molecular Mass: 36 kDa
• Protein length: Met1-Asp275
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ATG5 ATG5 autophagy related 5 homolog (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol: ATG5
• Synonyms: ATG5; ATG5 autophagy related 5 homolog (S. cerevisiae); APG5 (autophagy 5, S. cerevisiae) like, APG5 autophagy 5 like (S. cerevisiae), APG5L; autophagy protein 5; APG5; ASP; hAPG5; apoptosis specific protein; apoptosis-specific protein; APG5L; APG5-LIKE
• Gene ID: 9474
• mRNA Refseq: NM_004849
• Protein Refseq: NP_004840
• MIM: 604261
• UniProt ID: Q9H1Y0