Recombinant Human ATP6V1C1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ATP6V1C1-1161H
• Product Overview: ATP6V1C1 MS Standard C13 and N15-labeled recombinant protein (NP_001686) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 43.9 kDa
• AA Sequence: MTEFWLISAPGEKTCQQTWEKLHAA TSKNNNLAVTSKFNIPDLKVGTLDV LVGLSDELAKLDAFVEGVVKKVAQY MADVLEDSKDKVQENLLANGVDLVT YITRFQWDMAKYPIKQSLKNISEII AKGVTQIDNDLKSRASAYNNLKGNL QNLERKNAGSLLTRSLAEIVKKDDF VLDSEYLVTLLVVVPKLNHNDWIKQ YETLAEMVVPRSSNVLSEDQDSYLC NVTLFRKAVDDFRHKARENKFIVRD FQYNEEEMKADKEEMNRLSTDKKKQ FGPLVRWLKVNFSEAFIAWIHVKAL RVFVESVLRYGLPVNFQAMLLQPNK KTLKKLREVLHELYKHLDSSAAAII DAPMDIPGLNLSQQEYYPYVYYKID CNLLEFKTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ATP6V1C1 ATPase H+ transporting V1 subunit C1 [ Homo sapiens (human) ]
• Official Symbol: ATP6V1C1
• Synonyms: ATP6C; ATP6D; ATP6V1C1; ATPase H+ transporting lysosomal (vacuolar proton pump) 42kD; ATPase H+ transporting lysosomal 42kD V1 subunit C isoform 1; ATPase H+ transporting lysosomal 42kDa V1 subunit C isoform 1; ATPase H+ transporting lysosomal 42kDa V1 subunit C1; ATPase H+ transporting lysosomal V1 subunit C1; FLJ20057; H(+) transporting two sector ATPase subunit C; H+ ATPase C subunit; H+ transporting ATPase chain C vacuolar; Subunit C of vacuolar proton ATPase V1 domain; V ATPase C subunit; V ATPase subunit C 1; V-ATPase subunit C 1; V-type proton ATPase subunit C 1; Vacuolar ATP synthase subunit C; Vacuolar proton pump 42 kD subunit; Vacuolar proton pump C subunit; Vacuolar proton pump subunit C 1; Vacuolar protonATPase subunit C VI domain; VATC; VATC1_HUMAN; VATPase C subunit; VATPase subunit C 1; VMA5; ATP6V1C1
• Gene ID: 528
• mRNA Refseq: NM_001695
• Protein Refseq: NP_001686
• MIM: 603097
• UniProt ID: P21283