Recombinant Human AUH, His-tagged

• Cat.No.: AUH-27419TH
• Product Overview: Recombinant full length Human AUH with an N-terminal His tag; 293 amino acids with tag, MWt 31.4 kDa.

Specification

• Description: The methylglutaconyl-CoA hydratase, mitochondrial protein binds to the AU-rich element (ARE), a common element found in the 3 UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92% identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40-kDa precursor protein which is subsequently processed to a 32-kDa mature form.
• Protein length: 272 amino acids
• Conjugation: HIS
• Molecular Weight: 31.400kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMSSEM KTEDELRVRHLEEENRGIVVLGINR AYGKNSLSKNLIKMLSKAVDALKSD KKVRTIIIRSEVPGIFCAGADLKER AKMSSSEVGPFVSKIRAVINDIANL PVPTIAAIDGLALGGGLELALACDI RVAASSAKMGLVETKLAIIPGGGGT QRLPRAIGMSLAKELIFSARVLDGK EAKAVGLISHVLEQNQEGDAAYRKA LDLAREFLPQGPVAMRVAKLAINQG MEVDLVTGLAIEEACYAQTIPTKDR LEGLLAFKEKRPPRYKGE

Gene Information

• Gene Name: AUH AU RNA binding protein/enoyl-CoA hydratase [ Homo sapiens ]
• Official Symbol: AUH
• Synonyms: AUH; AU RNA binding protein/enoyl-CoA hydratase; AU RNA binding protein/enoyl Coenzyme A hydratase , AU RNA binding protein/enoyl Coenzyme A hydratase; methylglutaconyl-CoA hydratase, mitochondrial
• Gene ID: 549
• mRNA Refseq: NM_001698
• Protein Refseq: NP_001689
• MIM: 600529
• Uniprot ID: Q13825
• Chromosome Location: 9q22
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, organism-specific biosystem; Leucine degradation, leucine => acetoacetate + acetyl-CoA, conserved biosystem
• Function: RNA binding; enoyl-CoA hydratase activity; lyase activity; mRNA 3-UTR binding; methylglutaconyl-CoA hydratase activity