Recombinant Human Cadherin 2, Type 1, N-cadherin (Neuronal)

• Cat.No.: CDH2-87H
• Product Overview: Recombinant Human Cadherin-N protein 741-907 a.a. expressed inE.coli,43 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 741-907 a.a.
• Description: Cadherins comprise a family of Ca+ dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains.
• Presentation: Recombinant Human Cadherin-N protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
• Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 43 kDa.
• Applications: •ELISA •Inhibition Assays •Western Blotting
• Storage: Store vial at -20°Cto -80°C. When stored at the recommended temperature, this protein is stable for 12 months.

Gene Information

• Gene Name: CDH2 cadherin 2, type 1, N-cadherin (neuronal) [ Homo sapiens ]
• Synonyms: CDH2; cadherin 2, type 1, N-cadherin (neuronal); CDHN; NCAD; CD325; CDw325; cadherin 2, type 1; N-cadherin 1; neural cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; 5` partial; N-cadherin; Cadherin-2;CD325 antigen
• Gene ID: 1000
• mRNA Refseq: NM_001792
• Protein Refseq: NP_001783
• MIM: 114020
• UniProt ID: P19022
• Chromosome Location: 18q12.1
• Pathway: Arrhythmogenic right ventricular cardiomyopathy (ARVC); Cell adhesion molecules (CAMs); Cell-cell adhesion systems
• Function: RPTP-like protein binding; beta-catenin binding; calcium ion binding; protein kinase binding; protein phosphatase binding

Citation

Rickettsia Sca4 reduces vinculin-mediated intercellular tension to promote spread

Journal: Cell    PubMed ID: 27768890    Data: 2017/10/20

Authors: Rebecca L. Lamason, Effie Bastounis, Matthew D. Welch

Article Snippet:To measure traction forces mediated by E-cadherin, activated gels were first coated with 0.2 mg/ml rabbit anti-human IgG (Fcγ-fragment specific; Jackson ImmunoResearch) and incubated overnight at 4°C.To measure traction forces mediated by E-cadherin, activated gels were first coated with 0.2 mg/ml rabbit anti-human IgG (Fcγ-fragment specific; Jackson ImmunoResearch) and incubated overnight at 4°C.. Gels were washed with PBS the next day to remove unbound antibody and incubated for 3 h at 4°C with 50 μg/ml recombinant human E-cadherin/Fc chimera (Creative BioMart).. Gels were then washed with PBS, blocked for 30 min at room temperature with 1% BSA/PBS, washed again with PBS, and equilibrated in DMEM + 10% FBS for 30 min at 37°C prior to adding cells.Gels were then washed with PBS, blocked for 30 min at room temperature with 1% BSA/PBS, washed again with PBS, and equilibrated in DMEM + 10% FBS for 30 min at 37°C prior to adding cells.

TFM results for individual cells adherent to polyacrylamide gels coated with E-cadherin-Fc (A, B) or collagen I (C, D). (A, C) Instantaneous maps showing the magnitude of traction stresses (color indicates stress values in Pa). Cell outlines in white. (B, D) Time-averaged strain energy (nN μm) from individual cells. (E) Model depicting how biomechanical crosstalk allows measurements of traction stresses at cell-ECM junctions to be converted to tension. Cells adhere to a gel containing green fluorescent beads in upper layer. Actin cables (red) connect E-cadherin (orange) and integrin (blue) adhesions. Sca4 specifically targets cell-cell junctions. (F) MSM results for cell monolayers adherent to gels coated with collagen I. Images show the distribution of tension (right) and cells as seen by phase contrast (left). (G) Time-averaged monolayer tension (nN μm?1) calculated for multiple regions within the monolayer. Mann-Whitney rank sum T-test: * p < 0.05, ** p < 0.01, **** p < 0.0001. See Figure S2.