Recombinant Human CASC5 Protein, His&GST-tagged

• Cat.No.: CASC5-620H
• Product Overview: Recombinant Human CASC5 Protein (Met1-Ala277) with His&GST tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The encoded protein functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4). Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their biological validity has not been confirmed.
• Source: E. coli
• Species: Human
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 61.6 kDa; Accurate Molecular Mass: 62 kDa
• Protein length: Met1-Ala277
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: CASC5 cancer susceptibility candidate 5 [ Homo sapiens (human) ]
• Official Symbol: CASC5
• Synonyms: CASC5; cancer susceptibility candidate 5; protein CASC5; AF15Q14; blinkin; bub linking kinetochore protein; cancer/testis antigen 29; CT29; D40; hKNL 1; hSpc105; kinetochore null 1 homolog (C. elegans); KNL1; PPP1R55; protein phosphatase 1; regulatory subunit 55; kinetochore null 1 homolog; kinetochore-null protein 1; bub-linking kinetochore protein; blinkin, bub-linking kinetochore protein; protein phosphatase 1, regulatory subunit 55; ALL1-fused gene from chromosome 15q14 protein; cancer susceptibility candidate gene 5 protein; hKNL-1; KIAA1570
• Gene ID: 57082
• mRNA Refseq: NM_144508
• Protein Refseq: NP_653091
• MIM: 609173
• UniProt ID: Q8NG31