Active Recombinant Human CD47 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD47-556H
• Product Overview: Biotinylated Recombinant Human CD47 protein(Gln 19 - Pro 139), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Monoclonal Anti-Human CD47 Antibody, Human IgG4 at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, His,Avitag with a linear range of 0.1-3 ng/mL.Immobilized Human SIRP alpha, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, His,Avitag with a linear range of 0.2-25 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 16.9 kDa. The protein migrates as 32-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln19-Pro139
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 24 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD47 CD47 molecule [ Homo sapiens ]
• Official Symbol: CD47
• Synonyms: CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer) , MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6
• Gene ID: 961
• mRNA Refseq: NM_001777
• Protein Refseq: NP_001768
• MIM: 601028
• UniProt ID: Q08722