Active Recombinant Human CD47 Protein, hIgG/His-tagged

• Cat.No.: CD47-14H
• Product Overview: Recombinant human CD47 (19-141aa, 362aa), fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
• Source: Insect cell
• Species: Human
• Tag: His&Fc
• Form: Liquid
• Bio-activity: Measured by its binding ability in a functional ELISA with Human SIRP alpha/CD172a. The ED50 range ≤ 100 ng/mL.
• Molecular Mass: 40.9 kDa
• Protein length: 19-141
• AA Sequence: QLLFNKTKSVEFTFCNDTVVIPCFV TNMEAQNTTEVYVKWKFKGRDIYTF DGALNKSTVPTDFSSAKIEVSQLLK GDASLKMDKSDAVSHTGNYTCEVTE LTREGETIIELKYRVVSWFSPNE
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: CD47 CD47 molecule [ Homo sapiens (human) ]
• Official Symbol: CD47
• Synonyms: CD47; CD47 molecule; IAP; OA3; MER6; leukocyte surface antigen CD47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; Rh-related antigen; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; integrin associated protein; integrin-associated signal transducer
• Gene ID: 961
• mRNA Refseq: NM_001777
• Protein Refseq: NP_001768
• MIM: 601028
• UniProt ID: Q08722