||Human DAPK1 (NP_004929.1, 1 a.a. - 289 a.a.) partial recombinant protein with His tag expressed in Baculovirus infected Sf21 cells.
||Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
||The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.Substrate : DAPK1tide. ATP: 100 µM.
||94 % by SDS-PAGE/CBB staining.
||Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
||In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)