Active Recombinant Human DAPK1, GST-tagged

• Cat.No.: DAPK1-1395H
• Product Overview: Recombinant human DAPK1 (1-363) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-363 aa
• Description: Death-associated protein kinase 1 (DAPK1) is a positive mediator of apoptosis induced by γ-interferon. Activation of DAPK occurs via dephosphorylation of Ser-308 and subsequent association of calcium/calmodulin. DAPK is rapidly dephosphorylated in response to tumor necrosis factor or ceramide and then subsequently degraded via proteasome activity. The decline in DAPK expression is paralleled with increased caspase activity and cell apoptosis. Studies suggest that the apoptosis regulatory activities mediated by DAPK are controlled both by phosphorylation status and protein stability.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 49 nmol/min/mg
• Molecular Mass: ~71 kDa
• Purity: >80%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: DAPK1 death-associated protein kinase 1 [ Homo sapiens ]
• Official Symbol: DAPK1
• Synonyms: DAPK1; death-associated protein kinase 1; DAPK; DAP kinase 1; DKFZp781I035
• Gene ID: 1612
• mRNA Refseq: NM_004938
• Protein Refseq: NP_004929
• MIM: 600831
• UniProt ID: P53355
• Chromosome Location: 9q34.1
• Pathway: Apoptosis, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; IFN-gamma pathway, organism-specific biosystem; Pathways in cancer, organism-specific biosystem; Regulation of Apoptosis, organism-specific biosystem; Role of DCC in regulating apoptosis, organism-specific biosystem
• Function: ATP binding; calmodulin binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity