Recombinant Human DDX3Y Protein, GST-tagged

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Recombinant Human DDX3Y Protein, GST-tagged

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Cat.No. : DDX3Y-2484H
Product Overview : Human DDX3Y full-length ORF ( NP_004651.2, 1 a.a. - 660 a.a.) recombinant protein with GST-tag at N-terminal.
Description : The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 99.6 kDa
AA Sequence : MSHVVVKNDP ELDQQLANLD LNSEKQSGGA STASKGRYIP PHLRNREASK GFHDKDSSGW SCSKDKDAYS SFGSRDSRGK PGYFSERGSG SRGRFDDRGR SDYDGIGNRE RPGFGRFERS GHSRWCDKSV EDDWSKPLPP SERLEQELFS GGNTGINFEK YDDIPVEATG SNCPPHIENF SDIDMGEIIM GNIELTRYTR PTPVQKHAIP IIKGKRDLMA CAQTGSGKTA AFLLPILSQI YTDGPGEALK AVKENGRYGR RKQYPISLVL APTRELAVQI YEEARKFSYR SRVRPCVVYG GADIGQQIRD LERGCHLLVA TPGRLVDMME RGKIGLDFCK YLVLDEADRM LDMGFEPQIR RIVEQDTMPP KGVRHTMMFS ATFPKEIQML ARDFLDEYIF LAVGRVGSTS ENITQKVVWV EDLDKRSFLL DILGATGSDS LTLVFVETKK GADSLEDFLY HEGYACTSIH GDRSQRDREE ALHQFRSGKS PILVATAVAA RGLDISNVRH VINFDLPSDI EEYVHRIGRT GRVGNLGLAT SFFNEKNMNI TKDLLDLLVE AKQEVPSWLE NMAYEHHYKG GSRGRSKSNR FSGGFGARDY RQSSGSSSSG FGASRGSSSR SGGGGYGNSR GFGGGGYGGF YNSDGYGGNY NSQGVDWWGN
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing: :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : DDX3Y DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked [ Homo sapiens ]
Official Symbol : DDX3Y
Synonyms : DDX3Y; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked; DBY, DEAD/H (Asp Glu Ala Asp/His) box polypeptide, Y chromosome; ATP-dependent RNA helicase DDX3Y; DEAD box protein 3, Y-chromosomal; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, Y chromosome; DBY;
Gene ID : 8653
mRNA Refseq : NM_001122665
Protein Refseq : NP_001116137
MIM : 400010
UniProt ID : O15523

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