Recombinant Human DIABLO, T7 -tagged

• Cat.No.: DIABLO-30928TH
• Product Overview: Recombinant full length Human Smac / Diablo fused to a T7 tag at the N terminal; 199 amino acids, MWt 22 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Description: This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein.
• Conjugation: T7
• Tissue specificity: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20mM Tris, pH 7.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MASMTGGQQM GRGSMAVPIA QKSEPHSLSS EALMRRAVSL VTDSTSTFLS QTTYALIEAI TEYTKAVYTL TSLYRQYTSL LGKMNSEEED EVWQVIIGAR AEMTSKHQEY LKLETTWMTA VGLSEMAAEA AYQTGADQAS ITARNHIQLV KLQVEEVHQL SRKAETKLAE AQIEELRQKT QEEGEERAES EQEAYLRED
• Full Length: Full L.

Gene Information

• Gene Name: DIABLO diablo, IAP-binding mitochondrial protein [ Homo sapiens ]
• Official Symbol: DIABLO
• Synonyms: DIABLO; diablo, IAP-binding mitochondrial protein; diablo homolog, mitochondrial; DFNA64; DIABLO S; FLJ10537; FLJ25049; second mitochondria derived activator of caspase; SMAC
• Gene ID: 56616
• mRNA Refseq: NM_019887
• Protein Refseq: NP_063940
• MIM: 605219
• Uniprot ID: Q9NR28
• Chromosome Location: 12q24.31
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptotic factor-mediated response, organism-specific biosystem; Caspase cascade in apoptosis, organism-specific biosystem; Intrinsic Pathway for Apoptosis, organism-specific biosystem
• Function: protein binding

Citation

Discovery of Covalent MKK4/7 Dual Inhibitor.

Journal: Cell chemical biology    PubMed ID: 32916088    Data: 2021/7/5

Authors: Jie Jiang, Baishan Jiang, Nathanael S Gray

Article Snippet:REAGENT or RESOURCE SOURCE IDENTIFIER Mycoalert Lonza Lonza Cat # LT07-318 Recombinant Human MKK7 protein, His & T7-tagged Creative BioMart Cat # MAP2K7-8083H Recombinant Human MKK4, His-tagged Creative BioMart Cat # MAP2K4-105H Anisomycin Santa Cruz Biotechnology Cat# SC-3524 CellTiter Glo Promega Cat # G7571 Critical Commercial Assays Lanthascreen (MKK4) Thermo Fisher Scientific Assay ID 2209 Lanthascreen (MKK4)_5 min Thermo Fisher Scientific Assay ID 2543 Lanthascreen (MKK4)_20 min Thermo Fisher Scientific Assay ID 2544 Lanthascreen (MKK4)_40 min Thermo Fisher Scientific Assay ID 2545 Lanthascreen (MKK4)_90 min Thermo Fisher Scientific Assay ID 2547 Z’ Lyte (GSK3a) Thermo Fisher Scientific Assay ID 492 Z’ Lyte (GSK3b) Thermo Fisher Scientific Assay ID 495 32P-ATP kinase assay (MKK7) Reaction Biology N/A Mobility shift (MKK7) NanoSyn N/A KiNativ kinase panel profiling AcitvX N/A KINOMEscan DiscoverX Corp. N/A BCA protein assay kit Thermo Fisher Scientific Cat # 23227 Deposited Data MKK4 protein structure Matsumoto, et al., 2010 PDB: 3ALN MKK2 protein structure Ohren, et al., 2004 PDB: 1S9I Experimental Models: Cell Lines Human: MDA-MB-231 cells (female) ATCC Cat # HTB-26; RRID:CVCL_0062 Recombinant DNA MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA Sigma Aldrich Cat # SHC016 Software and Algorithms Prism Graphpad RRID:SCR_002798 Other Streptavidin-agarose beads Thermo Fisher Cat # 20349