Recombinant Human DPYS protein, His&GST-tagged

• Cat.No.: DPYS-0254H
• Product Overview: Recombinant Human DPYS protein(NP_001376.1)(Met1-Pro519), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant human DPYS/GST chimera consists of 756 amino acids and has a calculated molecular mass of 84.4 kDa. The recombinant protein migrates as an approximately 69 kDa band in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Pro519
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DPYS dihydropyrimidinase [ Homo sapiens ]
• Official Symbol: DPYS
• Synonyms: DPYS; dihydropyrimidinase; DHPase; hydantoinase; dihydropyrimidine amidohydrolase; DHP
• Gene ID: 1807
• mRNA Refseq: NM_001385
• Protein Refseq: NP_001376
• MIM: 613326
• UniProt ID: Q14117