Recombinant Human F7

• Cat.No.: F7-124H
• Product Overview: Recombinant Human Factor VIIa produced inBHKis a glycosylated polypeptide two-chain dimmer. The protein contains 406 amino acids and has a molecular weight of 50kD. The protein is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: BHK
• Tag: Non
• Description: This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of this gene results in 2 transcripts. Defects in this gene can cause coagulopathy.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Formulation: The protein 1mg/ml was lyophilized after from a sterile solution containing 4.86mg sodium chloride, 2.45mg calcium chloride dihydrate, 50mg mannitol and 116µg Tween 80.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
• Dimers And Aggregates: Less than 1% as determined by silver stained SDS-PAGE gel analysis.
• Biological Activity: The rHuFactor VIIa is fully biologically active when compared to standard. The potency per mg was tested and found to be 50,000Units/mg.
• Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuFactor-VIIa.
• Solubility: It is recommended to reconstitute the lyophilized rHuFactor-VIIa in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized rHuFactor-VIIa although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuFactor-VIIa should be stored at 4°Cbetween 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: F7 coagulation factor VII (serum prothrombin conversion accelerator) [ Homo sapiens ]
• Synonyms: F7; coagulation factor VII (serum prothrombin conversion accelerator); coagulation factor VII; eptacog alfa; FVII coagulation protein; factor VII;EC3.4.21.21; Serum prothrombin conversion accelerator; SPCA;Factor VII light chain; Factor VII heavy chain; OTTHUMP00000018733; OTTHUMP00000018734; Proconvertin; Eptacog alfa
• Gene ID: 2155
• mRNA Refseq: NM_000131
• Protein Refseq: NP_000122
• UniProt ID: P08709
• Chromosome Location: 13q34
• MIM: 227500
• Pathway: Complement and coagulation cascades; emostasis; Metabolism of proteins
• Function: calcium ion binding; glycoprotein binding; peptidase activity; serine-type endopeptidase activity