Recombinant Human Fas Ligand Inhibitor, Fc-tagged

• Cat.No.: FAS-37H
• Product Overview: Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1)(aa 7-154) is fused to the Fc portion of human IgG1. Purified by multi-step chromatography.

Specification

• Species: Human
• Tag: Fc
• Protein Length: 7-154 a.a.
• Form: 50 μg lyophilized, contains PBS.
• Molecular Mass: ~56 kDa
• Endotoxin: Endotoxin content is<0.1 eu/μg="" purified="" protein="" as="" determined="" by="" lal="" test.="">
• Purity: ≥95% as determined by SDS-PAGE.
• Characteristic: Inhibits the apoptosis-inducing activity of Fas Ligand. Binds FasL.
• Applications: Functional Application: Inhibition of FAS Ligand activity in human and mouse: The apoptosis-inducing activity of FasL is inhibited by concentrations of 20-100 μg/mL of the fusion protein.
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Storage: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles (activity may decrease).
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.

Gene Information

• Gene Name: FAS Fas (TNF receptor superfamily, member 6) [ Homo sapiens ]
• Official Symbol: FAS
• Synonyms: FAS; Fas (TNF receptor superfamily, member 6); APT1, FAS1, TNFRSF6, tumor necrosis factor receptor superfamily, member 6; tumor necrosis factor receptor superfamily member 6; APO 1; CD95; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; apoptosis-mediating surface antigen FAS; tumor necrosis factor receptor superfamily, member 6; APT1; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Gene ID: 355
• mRNA Refseq: NM_000043
• Protein Refseq: NP_000034
• MIM: 134637
• UniProt ID: P25445
• Chromosome Location: 10q24.1
• Pathway: Activation of Pro-Caspase 8, organism-specific biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Alzheimers disease, organism-specific biosystem
• Function: binding; identical protein binding; kinase binding; protein binding; receptor activity; receptor activity; signal transducer activity; transmembrane signaling receptor activity; tumor necrosis factor-activated receptor activity

Citation

Fascin Is Critical for the Maintenance of Breast Cancer Stem Cell Pool Predominantly via the Activation of the Notch Self-Renewal Pathway.

Journal: Stem cells (Dayton, Ohio)    PubMed ID: 27502039    Data: 2017/12/4

Authors: Rayanah Barnawi, Samiyah Al-Khaldi, Monther Al-Alwan

Article Snippet:The ALDEFLUOR? Kit which measure aldehyde dehydrogenase (ALDH) activity was purchased from StemCell Technologies.The ALDEFLUOR? Kit which measure aldehyde dehydrogenase (ALDH) activity was purchased from StemCell Technologies.. The recombinant human Notch ligand (DLLI) was purchased from Creative BioMart and the Notch signaling inhibitor (FLI-06) was purchased from Selleckchem.. Flow cytometry and cell sorting Cell surface staining and permeablization to detect fascin were performed as previously described [30].Flow cytometry and cell sorting Cell surface staining and permeablization to detect fascin were performed as previously described [30].