Recombinant Human Fas Ligand Inhibitor, Fc-tagged

• Cat.No.: FAS-37H
• Product Overview: Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1)(aa 7-154) is fused to the Fc portion of human IgG1. Purified by multi-step chromatography.

Specification

• Species: Human
• Tag: Fc
• Form: 50 μg lyophilized, contains PBS.
• Molecular Mass: ~56 kDa
• Endotoxin: Endotoxin content is<0.1 eu/μg purified protein as determined by lal test.>
• Purity: ≥95% as determined by SDS-PAGE.
• Characteristic: Inhibits the apoptosis-inducing activity of Fas Ligand. Binds FasL.
• Applications: Functional Application: Inhibition of FAS Ligand activity in human and mouse: The apoptosis-inducing activity of FasL is inhibited by concentrations of 20-100 μg/mL of the fusion protein.
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Storage: Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles (activity may decrease).
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.
• Protein length: 7-154

Gene Information

• Gene Name: FAS Fas (TNF receptor superfamily, member 6) [ Homo sapiens ]
• Official Symbol: FAS
• Synonyms: FAS; Fas (TNF receptor superfamily, member 6); APT1, FAS1, TNFRSF6, tumor necrosis factor receptor superfamily, member 6; tumor necrosis factor receptor superfamily member 6; APO 1; CD95; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; apoptosis-mediating surface antigen FAS; tumor necrosis factor receptor superfamily, member 6; APT1; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Gene ID: 355
• mRNA Refseq: NM_000043
• Protein Refseq: NP_000034
• MIM: 134637
• UniProt ID: P25445
• Chromosome Location: 10q24.1
• Pathway: Activation of Pro-Caspase 8, organism-specific biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Alzheimers disease, organism-specific biosystem
• Function: binding; identical protein binding; kinase binding; protein binding; receptor activity; receptor activity; signal transducer activity; transmembrane signaling receptor activity; tumor necrosis factor-activated receptor activity

Citation

Fascin Is Critical for the Maintenance of Breast Cancer Stem Cell Pool Predominantly via the Activation of the Notch Self-Renewal Pathway.

Journal: Stem cells (Dayton, Ohio)    PubMed ID: 27502039    Data: 2017/12/4

Authors: Rayanah Barnawi, Samiyah Al-Khaldi, Monther Al-Alwan

Article Snippet:The ALDEFLUOR? Kit which measure aldehyde dehydrogenase (ALDH) activity was purchased from StemCell Technologies.The ALDEFLUOR? Kit which measure aldehyde dehydrogenase (ALDH) activity was purchased from StemCell Technologies.. The recombinant human Notch ligand (DLLI) was purchased from Creative BioMart and the Notch signaling inhibitor (FLI-06) was purchased from Selleckchem.. Flow cytometry and cell sorting Cell surface staining and permeablization to detect fascin were performed as previously described [30].Flow cytometry and cell sorting Cell surface staining and permeablization to detect fascin were performed as previously described [30].

Reviews

09/07/2022

When experimenting with FAS, the results are very reproducible.

07/29/2022

The stability of FAS products allows them to cope with different experimental conditions and modes of operation.

09/20/2021

FAS has high specific activity and can exert strong biological effect at low concentration.

Q&As

Does FAS protein play a role in cell signaling?

FAS protein plays an important role in cell signaling, it can activate downstream signaling pathways, such as death signaling pathway and survival signaling pathway, by binding to its ligand and transmitting signals.

Why apply knowledge of FAS proteins to clinical practice?

It can provide a reference for clinical practice and guide the diagnosis, treatment and prevention of diseases by detecting the expression level of FAS protein and understanding its mechanism of action.

How can diseases associated with FAS protein be prevented and treated?

FAS protein-related diseases can be prevented and treated by regulating the expression level of FAS protein and inhibiting its signaling pathway.

How can gene knockout or knockdown techniques be used to study the function of FAS proteins?

Gene knockout or knockdown techniques can be used to study the function and mechanism of action of FAS proteins. By knocking out or knocking down the FAS gene, changes in processes such as apoptosis and immune response can be observed to further understand its function and mechanism of action.

What is the role of FAS protein in apoptosis?

FAS protein can bind to its ligand, activate the death signaling pathway, and induce apoptosis.

How can FAS protein be used to predict disease prognosis?

The expression level and activity of FAS protein can be measured to predict the prognosis and development trend of certain diseases.