Active Recombinant Human FCGR2B Protein, His & Avi-tagged, Biotinylated

• Cat.No.: FCGR2B-323H
• Product Overview: Recombinant Human FCGR2B Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 46 - Pro 217 (Accession # P31994-1). Predicted N-terminus: Ala 46. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 23.0 kDa. The protein migrates as 25 kDa and 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: His & Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Recombinant Human FCGR2B Protein, His & Avi-tagged, Biotinylated captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 5 μM as determined in a SPR assay (Biacore T200) (QC tested).
  Recombinant Human FCGR2B Protein, His & Avi-tagged, Biotinylated on SA Chip, can bind Rituximab with an affinity constant of 4.22 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Molecular Mass: 23.0 kDa
• Protein length: Ala 46 - Pro 217
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: FCGR2B Fc fragment of IgG, low affinity IIb, receptor (CD32) [ Homo sapiens ]
• Official Symbol: FCGR2B
• Synonyms: FCGR2B; CD32; CD32B; CDw32; fcRII-b; Fc gamma RIIb; FCG2; FCGR2; IGFR2
• Gene ID: 2213
• mRNA Refseq: NM_001002273
• Protein Refseq: NP_001002273
• MIM: 604590
• UniProt ID: P31994