Active Recombinant Human FCGR2B, His-tagged

• Cat.No.: FCGR2B-3820H
• Product Overview: A DNA sequence encoding the extracellular domain ( Ala 46 - Ile 224 ) of mature human CD32b protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Specification

• Description: The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene.
• Source: Human Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala 46
• Form: Lyophilized from sterile PBS , pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: Immobilized human CD32b-AVI-His at 10 μg/ml (100 μl/well) can bind biotinylated Human IgG1, The EC50 of biotinylated Human IgG1 is 0.20-0.48 μg/ml.
• Molecular Mass: The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 30-35 kDa protein due to glycosylation
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: FCGR2B Fc fragment of IgG, low affinity IIb, receptor (CD32) [ Homo sapiens (human) ]
• Official Symbol: FCGR2B
• Synonyms: Fc fragment of IgG, low affinity IIb, receptor (CD32); CD32; FCG2; CD32B; FCGR2; IGFR2; FCGR2B; Fc fragment of IgG, low affinity IIb, receptor; Fc gamma RIIb; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); CDw32; Fc-gamma-RIIb; FcRII-b; OTTHUMP00000032379; CD32 antigen; Fc-gamma RII-b; IgG Fc receptor II-b
• Gene ID: 2213
• mRNA Refseq: NM_001002274
• Protein Refseq: NP_001002274
• MIM: 604590
• UniProt ID: P31994
• Chromosome Location: 1q23
• Pathway: Adaptive Immune System; B cell receptor signaling pathway; BCR signaling pathway
• Function: IgG binding; protein binding