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Recombinant Human FCGRT & B2M Protein, His/Strep II-tagged, Biotinylated

Cat.No.: FCGRT & B2M
Product Overview: Biotinylated Human FcRn / FCGRT & B2M is expressed from HEK293 cells. It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # NP_001129491.1 (FCGRT) & AAH32589 (B2M)). The subunit FCGRT carries a polyhistidine tag at the C-terminus. The subunit B2M carries a Strep II tag at the C-terminus.
Source: HEK293
Species: Human
Tag : His/Strep II
Predicted N Terminal: Ala 24(FCGRT) & Ile 21(B2M)
Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity: Immobilized Yervoy Ipilimumab (Human IgG1) at 10 ug/mL (100 uL/well) can bind Biotinylated Human FcRn / FCGRT & B2M with a linear range of 0.02-0.15 ug/mL. Immobilized Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein on SA Biosensor, can bind Herceptin with an affinity constant of 0.49 uM as determined in BLI assay (Fortebio Octet 96).
Molecular Mass: The protein has a calculated MW of 31.2 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 33 kDa (FCGRT), 13 kDa (B2M) respectively on a SDS-PAGE gel under reducing condition due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Characteristic: The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product. The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Official Symbol: FCGRT; B2M
Synonyms: FcRn, FCGRT & B2M

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