Recombinant Human GFER, His-SUMO-tagged

• Cat.No.: GFER-216H
• Product Overview: Recombinant Human GFER /ALR MS Standard Protein, C13 and N15-labeled (GFER, Heavy Labeled) Met 81 - Asp 205 (Accession # NP_005253) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorporation eff

Specification

• Species: Human
• Source: HEK293
• Tag: His&SUMO
• Protein Length: 81-205 a.a.
• Description: Growth factor, augmenter of liver regeneration (GFER) is also known as FAD-linked sulfhydryl oxidase ALR, which belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. There are two isoform of GFER: Isoform 1 and isoform 2. Isoform 2 missing 1 – 80 aa. Isoform 1 is mainly located in mitochondrion intermembrane space, while Isoform 1 is secreted to cytoplasm. Isoform 1 of GFER regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. The isoform 2 of GFER may act as an autocrine hepatotrophic growth factor promoting liver regeneration.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: rh GFER, Heavy Labeled is fused with polyhistidine tag and SUMO tag at the N-terminus, and has a calculated MW of 27.3 kDa. The predicted N-terminus is Met 81. DTT-reduced Protein migrates as 33 kDa in SDS-PAGE .GFER, Heavy Labeled is labeled with [U- 13
• Endotoxin: Less than 1.0 EU per μg of the rh GFER, Heavy Labeled by the LAL method.
• Purity: >80% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: GFER growth factor, augmenter of liver regeneration [ Homo sapiens ]
• Official Symbol: GFER
• Synonyms: GFER; growth factor, augmenter of liver regeneration; growth factor, erv1 (S. cerevisiae) like (augmenter of liver regeneration); FAD-linked sulfhydryl oxidase ALR; ALR; ERV1; ERV1 homolog (S. cerevisiae); HERV1; HPO1; HPO2; HSS; ERV1 homolog; hepatopoietin protein; erv1-like growth factor; hepatic regenerative stimulation substance; HPO
• Gene ID: 2671
• mRNA Refseq: NM_005262
• Protein Refseq: NP_005253
• MIM: 600924
• UniProt ID: P55789
• Chromosome Location: 16p13.3-p13.12
• Pathway: Metabolism of proteins, organism-specific biosystem; Mitochondrial Protein Import, organism-specific biosystem
• Function: growth factor activity; oxidoreductase activity; protein binding; thiol oxidase activity