Recombinant Mouse Gfer Protein, MYC/DDK-tagged

• Cat.No.: Gfer-587M
• Product Overview: Purified recombinant protein of full-length mouse growth factor, augmenter of liver regeneration (Gfer), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen.
• Molecular Mass: 22.9 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gfer growth factor, augmenter of liver regeneration [ Mus musculus (house mouse) ]
• Official Symbol: Gfer
• Synonyms: Gfer; growth factor, augmenter of liver regeneration; Alr; ERV1; FAD-linked sulfhydryl oxidase ALR; augmenter of liver regeneration; growth factor, erv1-like (augmenter of liver regeneration); EC 1.8.3.2
• Gene ID: 11692
• mRNA Refseq: NM_023040
• Protein Refseq: NP_075527
• UniProt ID: P56213