ACTIVE Recombinant Human GZMA protein, His-tagged

• Cat.No.: GZMA-427H
• Product Overview: Recombinant Human GZMA protein(Ile29~Val262), fused with N-terminal His tag, was expressed in Yeast.

Specification

• Species: Human
• Source: Yeast
• Tag: His
• Tag: N-His
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Bio-activity: Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase independent cell death when introduced into target cells by perforin (1). Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1 26), a propeptide (residues 27-28) and a mature chain (residues 29-262 ) (2). The purified recombinant human Granzyme A consists of residues 29 to 262 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (Assay Buffer), initiated by addition 50 μL of various concentrations of GZMA (dilute by Assay Buffer) to 50 μL of 1.2 mM Substrate and DTNB mixture. The final well serves as a negative control with no GZMA, replace with 50μL assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme A is 912 pmol/min/μg.
• Molecular Mass: 28kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >98%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GZMA granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) [ Homo sapiens ]
• Official Symbol: GZMA
• Synonyms: GZMA; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); CTLA3, HFSP; granzyme A; CTL tryptase; Granzyme A (Cytotoxic T lymphocyte associated serine esterase 3; Hanukah factor serine protease); HF; h factor; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); HFSP; CTLA3
• Gene ID: 3001
• mRNA Refseq: NM_006144
• Protein Refseq: NP_006135
• MIM: 140050
• UniProt ID: P12544