Recombinant Human Heparanase

• Cat.No.: HPSE-213H
• Product Overview: Recombinant Human Heparanase-1 is produced as a latent 65 kDa precursor and proteolytically processed to its active form inCHO cell.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Description: Heparanase is an endo β-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor chance of survival.
• Concentration: 1µg /ml.
• Buffer: LDS-PAGE buffer
• Application: Positive control for western blot analysis.
• Storage: Store at –20 ℃, avoid repeated freeze-thaw cycles.
• Publications:
  Functional and structural characterization of a heparanase (2015)
  Metalloglycomics of tris(2,2′-bipyridyl) cobalt and ruthenium compounds (2022)

Gene Information

• Gene Name: HPSE heparanase [ Homo sapiens ]
• Synonyms: HPSE; heparanase; HPA; HPA1; HPR1; HPSE1; HSE1; heparanase-1; heparanase exon10-deletion; heparanase exon 9&10 deletion;EC=3.2.-.-; Heparanase 8 kDa subunit; Heparanase 50 kDa subunit
• Gene ID: 10855
• mRNA Refseq: NM_001098540
• Protein Refseq: NP_001092010
• MIM: 604724
• UniProt ID: Q9Y251
• Chromosome Location: 4q21.3
• Pathway: Glycosaminoglycan degradation; Metabolic pathways
• Function: beta-glucuronidase activity; calcium ion binding; hydrolase activity, acting on glycosyl bonds; magnesium ion binding

Citation

Functional and structural characterization of a heparanase.

Journal: Nature chemical biology    PubMed ID: 26565989    Data: 2016/4/12

Authors: Lisa Bohlmann, Gregory D Tredwell, Mark von Itzstein

Article Snippet:Heparan sulfate (100 μg) was incubated with enzyme (25 μg) at 37 °C (40 mM deuterated sodium acetate buffer, pH 5), and 1H NMR spectra were measured at the designated time points. (c) Cleavage of octasaccharide 5 by BpHepwt and human heparanase.Heparan sulfate (100 μg) was incubated with enzyme (25 μg) at 37 °C (40 mM deuterated sodium acetate buffer, pH 5), and 1H NMR spectra were measured at the designated time points. (c) Cleavage of octasaccharide 5 by BpHepwt and human heparanase.. Octasaccharide 5 (400 μM) was incubated at 37 °C with 1 μg of either BpHepwt in 40 mM deuterated sodium acetate buffer at pH 5 or human heparanase (Creative BioMart, USA) in 100 mM deuterated sodium acetate buffer at pH 4, and 1H NMR spectra were measured at designated time points.. Mass spectrometry analysis of BpHepwt digestion of octasaccharide 1.Mass spectrometry analysis of BpHepwt digestion of octasaccharide 1.