|Product Overview :
||Human HGF (heterodimer) (P14210, alpha chain 463 amino acids, beta chain 234 amino acids) recombinant protein expressed in Hi-5 cells.
||Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq
||The ED50 determined by the scattering activity in the MDCK cell assay is typically at 0.5-1.0 ng/mL.
|Molecular Mass :
||< 0.1 ng/μg
||>= 98% by SDS-PAGE and HPLC
||Store at -2 centigrade on dry atmosphere. After reconstitution with sterilized water, store at -2 centigrade. Aliquot to avoid repeated freezing and thawing.
|Storage Buffer :
||Lyophilized from 50 mM acetic acid