Recombinant Human HMGN2 protein, His-tagged

• Cat.No.: HMGN2-1445H
• Product Overview: Recombinant Human HMGN2 aa. (Pro2~Gln81) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Pro2~Gln81
• Description: The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi.
• Form: Freeze-dried powder
• Molecular Mass: 5kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >90%
• Characteristic: The isoelectric point is 10.2.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
  Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Note: It is better to add glycerol to keep stability after reconstituted.

Gene Information

• Gene Name: HMGN2 high mobility group nucleosomal binding domain 2 [ Homo sapiens (human) ]
• Official Symbol: HMGN2
• Synonyms: HMG17; high mobility group nucleosome-binding domain-containing protein 2; high mobility group protein N2; high-mobility group (nonhistone chromosomal) protein 17; nonhistone chromosomal protein HMG-17; non-histone chromosomal protein HMG-17
• Gene ID: 3151
• mRNA Refseq: NM_005517.3
• Protein Refseq: NP_005508.1
• UniProt ID: P05204