Recombinant Human HSPA5 Protein (Met1-Leu654), N-His tagged

• Cat.No.: HSPA5-3361H
• Product Overview: Recombinant human HSPA5 (Met1-Leu654) protein fused with the N-terminal His tag was expressed in E. coli.

Specification

• Description: Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1).
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Lyophilized
• Molecular Mass: 74.49 kDa
• Protein Length: Met1-Leu654
• Purity: >90 % as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4 % Trehalose, 1 % Mannitol.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) [ Homo sapiens (human) ]
• Official Symbol: HSPA5
• Synonyms: HSPA5; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); GRP78, heat shock 70kD protein 5 (glucose regulated protein, 78kD); 78 kDa glucose-regulated protein; BiP; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca (2+) -binding protein grp78; BIP; MIF2; GRP78; FLJ26106
• Gene ID: 3309
• mRNA Refseq: NM_005347
• Protein Refseq: NP_005338
• MIM: 138120
• UniProt ID: P11021