Active Recombinant Full Length Human HSPA5 Protein, C-Flag-tagged

• Cat.No.: HSPA5-52HFL
• Product Overview: Recombinant Full Length Human HSPA5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Protein refolding
  WB positive control
  Ubiquitination substrate
• Molecular Mass: 72.2 kDa
• AA Sequence: MKLSLVAAMLLLLSAARAEEEDKKE DVGTVVGIDLGTTYSCVGVFKNGRV EIIANDQGNRITPSYVAFTP EGERLIGDAAKNQLTSNPENTVFDA KRLIGRTWNDPSVQQDIKFLPFKVV EKKTKPYIQVDIGGGQTKTF APEEISAMVLTKMKETAEAYLGKKV THAVVTVPAYFNDAQRQATKDAGTI AGLNVMRIINEPTAAAIAYG LDKREGEKNILVFDLGGGTFDVSLL TIDNGVFEVVATNGDTHLGGEDFDQ RVMEHFIKLYKKKTGKDVRK DNRAVQKLRREVEKAKRALSSQHQA RIEIESFYEGEDFSETLTRAKFEEL NMDLFRSTMKPVQKVLEDSD LKKSDIDEIVLVGGSTRIPKIQQLV KEFFNGKEPSRGINPDEAVAYGAAV QAGVLSGDQDTGDLVLLDVC PLTLGIETVGGVMTKLIPRNTVVPT KKSQIFSTASDNQPTVTIKVYEGER PLTKDNHLLGTFDLTGIPPA PRGVPQIEVTFEIDVNGILRVTAED KGTGNKNKITITNDQNRLTPEEIER MVNDAEKFAEEDKKLKERID TRNELESYAYSLKNQIGDKEKLGGK LSSEDKETMEKAVEEKIEWLESHQD ADIEDFKAKKKELEEIVQPI
  ISKLYGSAGPPPTGEEDTAEKDELT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation, Prion diseases

Gene Information

• Gene Name: HSPA5 heat shock protein family A (Hsp70) member 5 [ Homo sapiens (human) ]
• Official Symbol: HSPA5
• Synonyms: BIP; GRP78; HEL-S-89n; MIF2
• Gene ID: 3309
• mRNA Refseq: NM_005347.5
• Protein Refseq: NP_005338.1
• MIM: 138120
• UniProt ID: P11021