Recombinant Human Human CDK1

• Cat.No.: CCNA1-31591TH
• Product Overview: Recombinant full length Human CDK1 and Cyclin-A1 with N-terminal proprietary tags on both proteins; MWt 58 and 81 kDa respectively.

Specification

• Description: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Baculovirus infected sf9 cells
• Tissue specificity: Isoform 2 is found in breast cancer tissues.
• Biological activity: The Specific activity of CCNA1-31591TH was determined to be 38 nmol/min/mg.
• Form: Liquid
• Purity: Densitometry
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.

Gene Information

• Gene Name: CCNA1 cyclin A1 [ Homo sapiens ]
• Official Symbol: CCNA1
• Synonyms: CCNA1; cyclin A1; cyclin-A1
• Gene ID: 8900
• mRNA Refseq: NM_001111046
• Protein Refseq: NP_001104516
• MIM: 604036
• Uniprot ID: P78396
• Chromosome Location: 13q12.3-q13
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem
• Function: protein binding; protein kinase binding