Recombinant Human IDH1 Protein, His-tagged
Cat.No. : | IDH1-023H |
Product Overview : | Recombinant Human IDH1 protein (2-414) with a C-terminal His-tag was expressed in E.coli |
- Specification
- Gene Information
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Description : | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Molecular Mass : | 47.9 kDa |
Protein length : | 2-414 |
Purity : | ≥95% estimated by SDS-PAGE |
Unit Definition : | One unit is defined as the amount of enzyme required to convert 1 nmol of NADPH to NADP +, using 15 mM α-ketoglutarate as a substrate, per minute at room temperature in 25 mM Tris-HCl, pH 7.5, 150 mM sodium chloride, and 5 mM MgCl2. |
Stability : | ≥ 6 months |
Storage : | -80 centigrade |
Storage Buffer : | 50 mM Tris-HCl, pH 7.5, containing 200 mM sodium chloride, 5 mM β-mercaptoethanol, and 10% glycerol |
Shipping : | Dry ice in continental US, may vary elsewhere. |
Gene Name : | IDH1 isocitrate dehydrogenase (NADP(+)) 1 [ Homo sapiens (human) ] |
Official Symbol : | IDH1 |
Synonyms : | IDH1; isocitrate dehydrogenase (NADP(+)) 1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; epididymis luminal protein 216; epididymis secretory protein Li 26; epididymis secretory sperm binding protein; isocitrate dehydrogenase (NADP(+)) 1, cytosolic; isocitrate dehydrogenase 1 (NADP+), soluble oxalosuccinate decarboxylase; EC 1.1.1.42 |
Gene ID : | 3417 |
mRNA Refseq : | NM_001282386 |
Protein Refseq : | NP_001269315 |
MIM : | 147700 |
UniProt ID : | O75874 |
Products Types
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IDH1-0133H | Recombinant Human IDH1 Protein (S2-L414), Tag Free | +Inquiry |
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IDH1-109H | Recombinant Human IDH1(R100A) Protein, His-tagged | +Inquiry |
◆ Lysates | ||
IDH1-5307HCL | Recombinant Human IDH1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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