Recombinant Human IDH1 Protein, His-tagged


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Recombinant Human IDH1 Protein, His-tagged

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Cat.No. : IDH1-023H
Product Overview : Recombinant Human IDH1 protein (2-414) with a C-terminal His-tag was expressed in E.coli
Description : Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 47.9 kDa
Protein length : 2-414
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to convert 1 nmol of NADPH to NADP +, using 15 mM α-ketoglutarate as a substrate, per minute at room temperature in 25 mM Tris-HCl, pH 7.5, 150 mM sodium chloride, and 5 mM MgCl2.
Stability : ≥ 6 months
Storage : -80 centigrade
Storage Buffer : 50 mM Tris-HCl, pH 7.5, containing 200 mM sodium chloride, 5 mM β-mercaptoethanol, and 10% glycerol
Shipping : Dry ice in continental US, may vary elsewhere.

SDS-PAGE Analysis of IDH1 :

Lane 1: MW Markers
Lane 2: IDH1 R123H (5 µg)
Lane 3: IDH1 R123H (2.5 µg)
Lane 4: IDH1 R123H (1.25 µg)

Gene Name : IDH1 isocitrate dehydrogenase (NADP(+)) 1 [ Homo sapiens (human) ]
Official Symbol : IDH1
Synonyms : IDH1; isocitrate dehydrogenase (NADP(+)) 1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; epididymis luminal protein 216; epididymis secretory protein Li 26; epididymis secretory sperm binding protein; isocitrate dehydrogenase (NADP(+)) 1, cytosolic; isocitrate dehydrogenase 1 (NADP+), soluble oxalosuccinate decarboxylase; EC
Gene ID : 3417
mRNA Refseq : NM_001282386
Protein Refseq : NP_001269315
MIM : 147700
UniProt ID : O75874
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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