Recombinant Human IL15RA 293 Cell Lysate
Cat.No. : | IL15RA-5247HCL |
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- Gene Information
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Description : | Antigen standard for interleukin 15 receptor, alpha (IL15RA), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | IL15RA interleukin 15 receptor, alpha [ Homo sapiens ] |
Official Symbol : | IL15RA |
Synonyms : | IL15RA; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; CD215; IL 15RA; MGC104179; |
Gene ID : | 3601 |
mRNA Refseq : | NM_002189 |
Protein Refseq : | NP_002180 |
MIM : | 601070 |
UniProt ID : | Q13261 |
Chromosome Location : | 10p15.1 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; |
Function : | cytokine receptor activity; protein binding; receptor activity; signal transducer activity; |
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◆ Lysates | ||
IL15RA-5246HCL | Recombinant Human IL15RA 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionImmunoprecipitation validates IL15RA's binding to IL2RB in cells.
CRISPR/Cas9 technology aids in generating IL15RA knockout models.
Approaches unravel IL15RA's implication in autoimmune disease mechanisms.
Methods discern IL15RA's distinct roles in innate and adaptive immunity.
RT-PCR and Western blotting assess IL15RA isoform expression patterns.
Binding affinity of IL15RA to IL15 quantified through surface plasmon resonance.
Proliferation assays investigate IL15RA's contribution to immune cell growth.
IL15RA's interaction with JAK/STAT pathways probed using immunoblotting.
siRNA knockdown studies unveil IL15RA's impact on downstream signaling.
FRET assays employed to elucidate IL15RA dimerization kinetics.
Customer Reviews (3)
Write a reviewEffective in T-cell assays.
High purity levels.
Consistent receptor binding.
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