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Recombinant Human IL18R1 Protein, His&GST-tagged

Cat.No. : IL18R1-1666H
Product Overview : Recombinant Human IL18R1 Protein (Tyr375-Glu428) with N-His&GST tag was expressed in E. coli.
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Description : The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Source : E. coli
Species : Human
Tag : N-His&GST
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 36.2 kDa
Accurate Molecular Mass: \
Protein length : Tyr375-Glu428
Purity : > 90%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : IL18R1 interleukin 18 receptor 1 [ Homo sapiens (human) ]
Official Symbol : IL18R1
Synonyms : IL18R1; interleukin 18 receptor 1; interleukin-18 receptor 1; CD218a; IL 1Rrp; IL1RRP; IL-18R1; IL1R-rp; IL-18R-1; cytokine receptor; IL1 receptor-related protein; CD218 antigen-like family member A; IL18RA; CDw218a; IL-1Rrp;
Gene ID : 8809
mRNA Refseq : NM_003855
Protein Refseq : NP_003846
MIM : 604494
UniProt ID : Q13478

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How do researchers study the co-receptor interactions involving IL18R1 and IL18RAP, and what methods are employed to decipher their cooperative signaling effects? 09/05/2022

Co-immunoprecipitation, co-receptor binding assays, and functional studies unveil the cooperative roles of IL18R1 and IL18RAP in signaling.

How are imaging techniques, such as fluorescence microscopy and live-cell imaging, applied to visualize the dynamics of IL18R1 localization and clustering upon IL18 stimulation? 04/09/2021

Fluorescence microscopy, confocal imaging, and super-resolution microscopy visualize IL18R1 dynamics and clustering upon IL18 stimulation.

What experimental methods are employed to investigate the post-translational modifications of IL18R1 and their impact on receptor function and downstream signaling? 10/05/2020

Mass spectrometry, phosphorylation assays, and ubiquitination studies investigate IL18R1 post-translational modifications.

How do researchers experimentally explore the role of IL18R1 in disease contexts, and what techniques are used to assess its potential as a therapeutic target? 08/05/2019

Disease model assessments, patient sample analyses, and pharmacological interventions explore IL18R1's involvement in diseases and therapeutic potential.

Could you explain the strategies used to assess the expression levels and tissue distribution of IL18R1 in different cell types and under varying physiological conditions? 04/26/2019

RT-qPCR, flow cytometry, and single-cell RNA sequencing assess IL18R1 expression across different cell types and physiological states.

Can you provide insights into the use of knockout mice, knockdown approaches, and gene editing techniques to elucidate the physiological importance of IL18R1? 03/14/2019

Knockout models, siRNA-mediated knockdown, and CRISPR/Cas9 gene editing reveal the physiological roles of IL18R1 in vivo and in vitro.

What cellular assays and functional studies are commonly utilized to investigate the downstream signaling cascades activated by IL18R1 upon IL18 binding? 09/19/2018

Western blotting, ELISAs, and phosphoprotein analyses decipher IL18R1-mediated signaling cascades and cytokine production.

What experimental approaches are commonly used to dissect the role of IL18R1's extracellular and intracellular domains in mediating IL18-induced immune responses? 06/24/2018

Site-directed mutagenesis, chimera studies, and domain swapping experiments dissect the functional contributions of IL18R1's domains.

Can you elaborate on the techniques used to investigate the structural basis of IL18R1's interaction with IL18 and its implications for downstream signaling? 10/04/2017

X-ray crystallography, cryo-electron microscopy, and molecular dynamics simulations elucidate the structural basis of IL18R1-IL18 recognition and activation.

How do researchers experimentally validate the binding affinity and kinetics of IL18R1 to IL18, considering their central role in initiating IL18-mediated signaling pathways? 09/07/2017

Surface plasmon resonance, bio-layer interferometry, and kinetic binding assays quantify IL18R1-IL18 interactions.

Customer Reviews (3)

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Reviews
03/07/2020

    Low endotoxin levels.

    03/15/2018

      Good for flow cytometry.

      09/06/2017

        Potent in Th1 activation.

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