Active Recombinant Human IL18R1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: IL18R1-1862H
• Product Overview: Biotinylated Recombinant Human IL18R1 protein(Ala 19 - Arg 329 ), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Biotinylated Human IL-18 R1, His,Avitag captured on Biotin CAP-Series S Sensor Chip can bind Human IL-18, Tag Free with an affinity constant of 1.49 μM as determined in a SPR assay (Biacore 8K).Biotinylated Human IL-18 R1, His,Avitag captured on Biotin CAP-Series S Sensor Chip can bind Human IL-18, Tag Free (Premixed with Human IL-18 R beta / IL-1 R7, Fc Tag, with an affinity constant of 5.87 nM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 39.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala19-Arg329
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL18R1 interleukin 18 receptor 1 [ Homo sapiens ]
• Official Symbol: IL18R1
• Synonyms: IL18R1; interleukin 18 receptor 1; interleukin-18 receptor 1; CD218a; IL 1Rrp; IL1RRP; IL-18R1; IL1R-rp; IL-18R-1; cytokine receptor; IL1 receptor-related protein; CD218 antigen-like family member A; IL18RA; CDw218a; IL-1Rrp
• Gene ID: 8809
• mRNA Refseq: NM_003855
• Protein Refseq: NP_003846
• MIM: 604494
• UniProt ID: Q13478