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Recombinant Human IL1A(Ser113-Ala271) Protein, C-Avi-tagged, Biotinylated

Cat.No. : IL1A-05H
Product Overview : Recombinant Human IL1A(P01583)(Ser113-Ala271) Protein, fused with a Avi tag at the C-terminus, was produced in E.coli. The purified protein was biotinylated in vitro.
  • Specification
  • Gene Information
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Source : E.coli
Species : Human
Tag : Avi
Form : Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Molecular Mass : The recombinant Human IL1A Protein has a predicted molecular mass of 20kDa, it migrates with an apparent molecular mass of 19kDa under reducing conditions
AA Sequence : Ser113-Ala271
Endotoxin : < 0.01 EU per μg of the protein as determined by the LAL method.
Purity : Greater than 95% as determined by reducing SDS-PAGE.
Characteristic : The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : IL1A interleukin 1, alpha [ Homo sapiens ]
Official Symbol : IL1A
Synonyms : IL1A; interleukin 1, alpha; IL1; interleukin-1 alpha; hematopoietin 1; IL 1A; IL1 ALPHA; IL1F1; preinterleukin 1 alpha; pro interleukin 1 alpha; IL-1 alpha; hematopoietin-1; pro-interleukin-1-alpha; IL-1A; IL1-ALPHA;
Gene ID : 3552
mRNA Refseq : NM_000575
Protein Refseq : NP_000566
MIM : 147760
UniProt ID : P01583

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What methods are employed to dissect the intricate cross-talk between IL-1α and other cytokines, shedding light on its role in immune modulation? 11/02/2021

Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.

What experimental approaches allow researchers to dissect the paradoxical roles of IL-1α as both a pro-inflammatory and tissue repair mediator in distinct contexts? 09/22/2021

IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.

How are cutting-edge techniques like single-cell RNA sequencing utilized to unravel the heterogeneity in IL-1α-responsive cell populations and their functional implications? 06/07/2021

Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.

How is the intricate web of IL-1α's pro-inflammatory signaling pathways decoded using advanced experimental techniques? 05/26/2021

IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.

How are state-of-the-art genetic editing tools such as CRISPR-Cas9 applied to unravel IL-1α's precise contributions to inflammatory diseases? 04/06/2021

Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.

How do researchers manipulate in vitro co-culture systems to dissect the dynamic interplay between immune cells and stromal cells in IL-1α-mediated responses? 06/18/2020

In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.

What experimental strategies enable the identification of specific cellular receptors and downstream effectors responsible for IL-1α's diverse physiological effects? 03/19/2020

Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.

How are sophisticated imaging techniques like live-cell microscopy harnessed to capture the real-time dynamics of IL-1α release and its impact on neighboring cells? 12/02/2019

Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.

How is the quantitative relationship between IL-1α levels and disease progression established through longitudinal studies and statistical modeling? 07/15/2018

Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.

How do multi-omics analyses, including transcriptomics and proteomics, provide a holistic view of the network of molecules influenced by IL-1α signaling? 06/04/2018

Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.

Customer Reviews (3)

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Reviews
04/08/2021

    High solubility.

    07/02/2020

      Good for intracellular staining.

      03/13/2019

        Effective in apoptosis assays.

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