| Description : |
Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to the cytokine IL-2. The IL-2R is made up of 3 subunits - alpha (α), beta (β) and gamma (γ). The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. The β and γ chains of the IL-2R are members of the type I cytokine receptor family. IL-2R has a high binding affinity to IL-2 and is expressed by antigen-activated T lymphocytes (T cells). IL-2 Rα is also known as CD25, p55, and Tac (activated T cell) antigen. |
| Source : |
HEK 293 |
| Species : |
Human |
| Tag : |
His |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1 ng/mL of recombinant IL-2, ED50 for this effect is < 1.5 μg/mL. |
| Molecular Mass : |
42 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
205 |
| AA Sequence : |
HHHHHHHHELCDDDPPEIPHATFKA MAYKEGTMLNCECKRGFRRIKSGSL YMLCTGNSSHSSWDNQCQCTSSATR NTTKQVTPQPEEQKERKTTEMQSPM QPVDQASLPGHCREPPPWENEATER IYHFVVGQMVYYQCVQGYRALHRGP AESVCKMTHGKTRWTQPQLICTGEM ETSQFPGEEKPQASPEGRPESETSC |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant Human Interleukin-2 Receptor α remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Human Interleukin-2 Receptor α should be stable up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
