Recombinant Cynomolgus IL2RA cell lysate
Cat.No. : | IL2RA-1015CCL |
Product Overview : | Cynomolgus IL2RA derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Cynomolgus |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | IL2RA interleukin 2 receptor, alpha [ Macaca fascicularis ] |
Official Symbol : | IL2RA |
Synonyms : | interleukin 2 receptor, alpha; interleukin 2 receptor alpha |
mRNA Refseq : | NM_001283704 |
Protein Refseq : | NP_001270633 |
Chromosome Location : | chromosome: 9 |
Pathway : | Cytokine-cytokine receptor interaction, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewSecurity is very high and can be used with confidence.
Structure is very stable and not prone to mutation.
The amount of expression is very high and can be easily detected.
Q&As (6)
Ask a questionThe combination of IL2RA and other drugs may produce nausea, vomiting, rash and other adverse reactions, should be monitored.
The frequency of use of IL2RA depends on the specific situation of the patient, and is generally used 1-3 times per week.
IL2RA, in the right dosage and frequency of use, can significantly relieve symptoms of diseases such as autoimmune diseases and organ transplant rejection.
Can assist in the treatment of some types of malignant tumors, but it is not a complete cure for cancer.
The effective rate of IL2RA therapy depends on many factors such as the type of disease, the patient's condition and the treatment regimen, and the effective rate is generally between 50% and 90%.
Indications of IL2RA include a variety of autoimmune diseases, organ transplant rejection and malignant tumors.
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