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Active Recombinant Human IL4 Protein (129 aa)

Cat.No. : IL4-244I
Product Overview : Recombinant human IL-4 (rhIL-4) is a 129 amino acid protein expressed in mammalian CHO system. The approximate molecular weight is 15 kDa analyzed by non-reducing SDS-PAGE.
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Description : Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet.
Source : CHO
Species : Human
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : ED50< 0.25 ng/mL, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of >4 6 units/mg
Molecular Mass : 15 kDa, observed by non-reducing SDS-PAGE.
Protein Length : 129
AA Sequence : HKCDITLQEIIKTLNSLTEQKTLCT ELTVTDIFAASKNTTEKETFCRAAT VLRQFYSHHEKDTRCLGATAQQFHR HKQLIRFLKRLDRNLWGLAGLNSCP VKEANQSTLENFLERLKTIMREKYS KCSS
Endotoxin : < 0.2 EU/μg, determined by LAL method.
Purity : > 95% as analyzed by SDS-PAGE and HPLC.
Storage : Lyophilized recombinant human Interleukin 4 (IL-4) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhIL-4 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade。
Storage Buffer : Lyophilized after extensive dialysis against PBS.
Reconstitution : Reconstituted in ddH2O or PBS at 100 μg/mL.
Gene Name : IL4 interleukin 4 [ Homo sapiens ]
Official Symbol : IL4
Synonyms : IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1;
Gene ID : 3565
mRNA Refseq : NM_000589
Protein Refseq : NP_000580
MIM : 147780
UniProt ID : P05112

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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10/17/2021

    By testing immunogenicity in animal models, detect whether an immune response is induced.

    11/09/2020

      The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

      05/06/2020

        This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.

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