Active Recombinant Human IL4 Protein (His25-Ser153), GMP Grade, Animal-Free
Cat.No. : | IL4-25HG |
Product Overview : | GMP recombinant human IL-4 protein (His25-Ser153) with an N-terminal Met was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines. |
- Specification
- Gene Information
- Related Products
Description : | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
Source : | E. coli |
Species : | Human |
Bio-activity : | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of recombinant human IL-4 is >1.0 x 107 IU/mg, which is calibrated against the human IL-4 WHO International Standard. |
Molecular Mass : | Predicted Molecular Mass: 15.1 kDa SDS-PAGE: 14 kDa, reducing conditions |
Protein Length : | His25-Ser153 |
N-terminal Sequence Analysis : | Met-His25-Lys-(Cys)-Asp-Ile-Thr-Leu-Gln-Glu |
Endotoxin : | <0.10 EU/ μg of the protein by the LAL method. |
Purity : | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, ≤ -20 centigrade under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution : | Reconstitute at 100-200 μg/mL in PBS. |
Shipping : | The product is shipped with polar packs. |
Gene Name : | IL4 interleukin 4 [ Homo sapiens ] |
Official Symbol : | IL4 |
Synonyms : | IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1; |
Gene ID : | 3565 |
mRNA Refseq : | NM_000589 |
Protein Refseq : | NP_000580 |
MIM : | 147780 |
UniProt ID : | P05112 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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