Recombinant Human IL5

• Cat.No.: IL5-125H
• Product Overview: Recombinant Human interleukin 5 (colony-stimulating factor, eosinophil) sequence (containing the signal peptide sequence, and the mature human Interleukin 5 sequence) was expressed in modifiedhuman 293 cells.

Specification

• Cat. No.: IL5-125H
• Description: Interleukin 5 (IL-5) plays an important role in B cell development by inducing the terminal differentiation of late-developing B cells. Additionally, IL-5 regulates the differentiation, survival and degranulation of eosinophils. IL-5 is expressed predominately by T-lymphocytes and mast cells, and to a lesser extent by eosinophils, natural killer cells and endothelial cells.
• Source: Human 293 cells.
• Amino Acid Sequence: IPTEIPTSALVK ETLALLSTHR TLLIANET LRIPVPVH KNHQLCTEE IFQGIGTLES QTVQGG TVE RL FKN LSLI K KYIDGQKK KCGEERRRV NQFLDYLQE FLG VMN TEWIIES.
• Molecular Mass: IL-5 migrates as a broad band between 15 and 20 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-5 that has a predicted molecular mass of 13.1 kDa.
• PI: IL-5 separates into a number of isoforms with a pI between 4.8 and 7.7 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-5 that has a predicted pI of 7.02.
• % Carbohydrate: Purified IL-5 consists of 10-35% carbohydrate by weight.
• Glycosylation: IL-5 has N-linked and O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Gene Information

• Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
• Synonyms: IL5; interleukin 5 (colony-stimulating factor, eosinophil); EDF; IL-5; TRF; B cell differentiation factor I; T-cell replacing factor; eosinophil differentiation factor; interleukin 5; interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; OTTHUMP00000065830
• Gene ID: 3567
• mRNA Refseq: NM_000879
• Protein Refseq: NP_000870
• MIM: 147850
• UniProt ID: P05113
• Chromosome Location: 5q31.1
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Hematopoietic cell lineage; Allograft rejection; Asthma; Autoimmune thyroid disease; Fc epsilon RI signaling pathway; T cell receptor signaling pathway
• Function: interleukin-5 receptor activity; cytokine activity; growth factor activity