Recombinant Human MGLL Protein, His-tagged


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Recombinant Human MGLL Protein, His-tagged

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Cat.No. : MGLL-39H
Product Overview : Recombinant human MGLL(2-313) protein with a C-terminal His-tag was expressed in E. coli.
Description : This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 39 kDa
Protein length : 2-313
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit of enzyme will produce 1 μmol of 4-nitrophenol per minute at 25 centigrade in 10 mM Tris, pH 7.2, 1 mM EDTA using 1.5 μM 4-nitrophenylacetate as substrate, measured by absorbance at 412 nm.
Stability : ≥ 2 years
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM HEPES, pH 7.4, 100 mM sodium chloride, 5 mM magnesium cloride, 0.1% Triton, and 25% glycerol
SDS-PAGE Analysis of MGLL :

Lane 1: MW Markers; Lane 2: MAGL (1 μg); Lane 3: MAGL (2 μg); Lane 4: MAGL (5 μg). Representative gel image shown; actual purity may vary between each batch.

Activity Assay :

MAGL activity was determined using 250 μM 4-nitrophenylacetate (4-NPA) and was inhibited by 1 μM methyl arachidonyl fluorophosphonate (MAFP), a general serine hydrolase inhibitor.

Gene Name : MGLL monoglyceride lipase [ Homo sapiens (human) ]
Official Symbol : MGLL
Synonyms : MGLL; monoglyceride lipase; MGL; HUK5; MAGL; HU-K5; monoglyceride lipase; lysophospholipase homolog; monoacylglycerol lipase
Gene ID : 11343
mRNA Refseq : NM_001003794
Protein Refseq : NP_001003794
MIM : 609699
UniProt ID : Q99685
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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