Recombinant Human MGLL Protein, His-tagged MGLL-39H

Recombinant Human MGLL Protein, His-tagged

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Recombinant Human MGLL Protein, His-tagged

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Cat.No. : MGLL-39H
Product Overview : Recombinant human MGLL(2-313) protein with a C-terminal His-tag was expressed in E. coli.
Description : This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 39 kDa
Protein length : 2-313
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit of enzyme will produce 1 μmol of 4-nitrophenol per minute at 25 centigrade in 10 mM Tris, pH 7.2, 1 mM EDTA using 1.5 μM 4-nitrophenylacetate as substrate, measured by absorbance at 412 nm.
Stability : ≥ 2 years
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM HEPES, pH 7.4, 100 mM sodium chloride, 5 mM magnesium cloride, 0.1% Triton, and 25% glycerol
SDS-PAGE Analysis of MGLL :

Lane 1: MW Markers; Lane 2: MAGL (1 μg); Lane 3: MAGL (2 μg); Lane 4: MAGL (5 μg). Representative gel image shown; actual purity may vary between each batch.

Activity Assay :

MAGL activity was determined using 250 μM 4-nitrophenylacetate (4-NPA) and was inhibited by 1 μM methyl arachidonyl fluorophosphonate (MAFP), a general serine hydrolase inhibitor.

Gene Name : MGLL monoglyceride lipase [ Homo sapiens (human) ]
Official Symbol : MGLL
Synonyms : MGLL; monoglyceride lipase; MGL; HUK5; MAGL; HU-K5; monoglyceride lipase; lysophospholipase homolog; monoacylglycerol lipase
Gene ID : 11343
mRNA Refseq : NM_001003794
Protein Refseq : NP_001003794
MIM : 609699
UniProt ID : Q99685

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