Recombinant Human MIF Protein MIF-018H

Recombinant Human MIF Protein

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Recombinant Human MIF Protein

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Cat.No. : MIF-018H
Product Overview : Recombinant Human MIF protein without tag was expressed in E.coli
Description : This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
Source : E. coli
Species : Human
Molecular Mass : 12.6 kDa
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to tautomerize 1 nmole of L-dopachrome (5,6-dihydroxyindole-2-carboxylate methyl ester) per minute at 21 centigrade in 40 mM Bis-Tris, pH 6.2, 0.8 mM EDTA, containing 260 μM L-dopachrome methyl ester, and 510 μM sodium periodate.
Stability : ≥ 6 months
Storage : -80 centigrade
Shipping : Dry ice in continental US, may vary elsewhere.
MIFcatalyzed decrease in dopachrome :

Purified MIF tautomerizes L-dopachrome, leading to a loss ofabsorbance at 475 nm.

SDS-PAGE Analysis of MIF :

Lane 1: MIF (2 µg)
Lane 2: MIF (5 µg)
Lane 3: MIF (10 µg)
Lane 4: MW Markers

Gene Name : MIF macrophage migration inhibitory factor [ Homo sapiens (human) ]
Official Symbol : MIF
Synonyms : MIF; macrophage migration inhibitory factor; GIF; GLIF; MMIF; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; epididymis secretory sperm binding protein; macrophage migration inhibitory factor (glycosylation-inhibiting factor); phenylpyruvate tautomerase; EC 5.3.2.1
Gene ID : 4282,
mRNA Refseq : NM_002415
Protein Refseq : NP_002406
MIM : 153620
UniProt ID : P14174

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