Recombinant Human Monoglyceride Lipase, His-tagged

• Cat.No.: MGLL-2863H
• Product Overview: Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 36.4 kDa(333aa), confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP
• Purity: > 85% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: MGLL monoglyceride lipase [ Homo sapiens ]
• Synonyms: MGLL; monoglyceride lipase; MGL; HUK5; MAGL; HU-K5; lysophospholipase-like; monoacylglycerol lipase; lysophospholipase homolog; EC 3.1.1.23
• Gene ID: 11343
• mRNA Refseq: NM_001003794
• Protein Refseq: NP_001003794
• MIM: 609699
• UniProt ID: Q99685
• Chromosome Location: 3q21.3
• Pathway: Glycerolipid metabolism; Metabolic pathways
• Function: acylglycerol lipase activity; hydrolase activity; lysophospholipase activity; protein homodimerization activity

Citation

Synthetic fluorogenic peptides reveal dynamic substrate specificity of depalmitoylases

Journal: Cell chemical biology    PubMed ID: 30393067    Data: 2020/1/17

Authors: Neri Amara, Ian T. Foe, Matthew Bogyo

Article Snippet:? REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-GAPDH clone 6C5 Millipore MAB374 Chemicals, Peptides, and Recombinant Proteins Recombinant T. gondii TgPPT1 W ( Child et al., 2013 ) N/A Recombinant T. gondii TgPPT1 S128A ( Child et al., 2013 ) N/A Recombinant human HsAPT1 WI ( Child et al., 2013 ; Garland et al., 2018 ) N/A Recombinant human HsAPT2 WI ( Child et al., 2013 ; Garland et al., 2018 ) N/A Recombinant phospholipase A2 (PLA2) EC 3.1.1.4 Sigma Aldrich Cat#P6534 Recombinant trypsin EC 3.4.21.4 Sigma Aldrich Cat#T 1426 Recombinant papain EC 3.4.22.2 Sigma Aldrich Cat#P4762 Recombinant monoacylglycerol lipase (MGLL) EC 3.1.1.23 Creative BioMart Cat#MGLL-2863H Collagenase IV EC 3.4.24.3 Worthington Biochemical Corp. Cat# {"type":"entrez-nucleotide","attrs":{"text":"LS004186","term_id":"1321650538","term_text":"LS004186"}} LS004186 Hexadecylfluorophosphonate HDFP This paper N/A JCP174 ( Child et al., 2013 ) N/A Palmostatin B Sigma Aldrich Cat#178501 ML348 ( Adibekian et al.,2010 ) N/A ML349 ( Adibekian et al.,2010 ) N/A fluorophosphonate-rhodamine (FP-Rho) ( Lentz et al., 2016 ) N/A hexadecylsulfonyl fluoride (HDSF) Santa Cruz Biotech.. Cat# sc-221708 CAS# 86855–26-7 phenylmethylsulfonyl fluoride (PMSF) Sigma Aldrich Cat# P7626 CAS#329–98-6 4-nitropehyl octanoate (4-NPO) Sigma Aldrich Cat#21742–1G-F CAS#1956–10-1 Quenched substrate for thioesterases QStE This paper N/A Quenched substrate for esterases QSE This paper N/A Critical Commercial Assays BCA protein assay kit Pierce Cat# 23225 Experimental Models: Cell Lines Human: ovary adenocarcinoma cell line SKOV3 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC HTB-77 Human: prostate adenocarcinoma cell line PC-3 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC CRL-1435 Human: prostate carcinoma cell line LnCaP ( Child et al., 2013 ; Garland et al., 2018 ) ATCC CRL-1740 Human: ovary adenocarcinoma cell line OVCAR-3 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC HTB-161 Human: breast adenocarcinoma cell line MDA-MB-231 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC HTB-26 Human: breast adenocarcinoma cell line MCF7 ( Child et al., 2013 ; Garland et al., 2018 ) ATCC HTB-22 Experimental Models: Organisms/Strains T. gondii strain ΔKu80 ( Child et al., 2013 ) N/A T. gondii strain ΔKu80Δppt1 ( Child et al., 2013 ) N/A Mouse: C57BL/6 MMTV-PyMT strain B6.FVB- Tg(MMTV-PyVT)634Mul/LellJ) Jackson Laboratories 022974 Other Syro II automated parallel peptide synthesizer Biotage N/A HPLC 1260 Infinity System Agilent Technologies N/A Finnigan LTQ mass detector Thermo Fischer Sci.

KEY RESOURCE TABLE