| Description : |
Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human Nectin is identical to the poliovirus receptor-related protein (PRR) and is identified to be the α herpesvirus entry mediator. Nectin constitutes a family consisting of at least Nectin 1, 2 and 3. Nectin 2 and 3 are ubiquitously expressed, whereas Nectin 1 is abundantly expressed in the brain. Nectin 1 exists as Nectin 1α and 1β/HIgR, produced by alternative splicing. The cytoplasmic regions of Nectin 1α, but not Nectin 1β/ HIgR, have a C-terminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein, afadin, through which it is linked to the Actin cytoskeleton. Nectin 1, also designated HveC/ PRR1, allows the entry of herpes simplex virus type 1 (HSV-1) and HSV-2 into mammalian cells. The interaction of virus envelope glycoprotein D (gD) with Nectin 1 is an essential step in the process leading to membrane fusion; the gD binding site is located at the first Ig-like domain of Nectin 1. Both the transinteraction of Nectin and the interaction of Nectin with afadin are necessary for their co-localization with E-cadherin and catenins at adherens junctions. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
C-His |
| Molecular Mass : |
~36 kDa |
| AA Sequence : |
QVVQVNDSMYGFIGTDVVLHCSFAN PLPSVKITQVTWQKSTNGSKQNVAI YNPSMGVSVLAPYRERVEFLRPSFT DGTIRLSRLELEDEGVYICEFATFP TGNRESQLNLTVMAKPTNWIEGTQA VLRAKKGQDDKVLVATCTSANGKPP SVVSWETRLKGEAEYQEIRNPNGTV TVISRYRLVPSREAHQQSLACIVNY HMDRFKESLTLNVQYEPEVTIEGFD GNWYLQRMDVKLTCKADANPPATEY HWTTLNGSLPKGVEAQNRTLFFKGP INYSLAGTYICEATNPIGTRSGQVE VNITEFPYTPSPPEHGRRAGPVPTA |
| Purity : |
Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE). |
| Notes : |
For research use only, not for use in diagnostic procedure. |
| Storage : |
Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles. |
| Concentration : |
≥0.5 mg/mL |
| Storage Buffer : |
PBS, 4M Urea, pH7.4 |
