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Recombinant Human NEFL Protein, His-tagged

Cat.No. : NEFL-2484H
Product Overview : Recombinant Human NEFL Protein (Lys281-Glu396) with a N-His tag was expressed in E. coli.
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Description : Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.
Source : E. coli
Species : Human
Tag : N-His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 17.0 kDa
Accurate Molecular Mass: \
Protein length : Lys281-Glu396
Endotoxin : <1.0 EU per 1µg (determined by the LAL method).
Purity : > 90%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Storage Buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : NEFL neurofilament, light polypeptide [ Homo sapiens (human) ]
Official Symbol : NEFL
Synonyms : NEFL; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament light polypeptide; CMT1F; CMT2E; NF68; NFL; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; light molecular weight neurofilament protein; NF-L; FLJ53642;
Gene ID : 4747
mRNA Refseq : NM_006158
Protein Refseq : NP_006149
MIM : 162280
UniProt ID : P07196

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the relationship between NEFL and neurofilaments? 02/26/2021

NEFL is the light chain subunit of neurofilaments, crucial for their assembly and function.

How does NEFL contribute to the stability and structure of axons? 08/01/2020

It is a major component of neurofilaments, providing stability and support to axons.

Are there known genetic mutations in NEFL associated with specific neurological conditions? 12/21/2019

Genetic mutations in NEFL have been linked to Charcot-Marie-Tooth disease and other neuropathies.

What is the primary function of NEFL in neuronal cells? 04/05/2019

NEFL plays a crucial role in maintaining the structure and function of neuronal axons.

In which cells or tissues is NEFL predominantly expressed? 12/17/2018

NEFL is predominantly expressed in neurons, particularly in the axons.

What therapeutic strategies are being explored involving NEFL for neurodegenerative diseases? 07/27/2018

Therapeutic strategies are being developed to target NEFL aggregation and dysfunction in neurodegenerative diseases.

How is NEFL implicated in neurodegenerative diseases? 05/13/2018

Abnormal accumulation of NEFL is associated with various neurodegenerative diseases.

Customer Reviews (3)

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Reviews
11/30/2022

    Worked well, fast shipping.

    06/06/2022

      Satisfied with the quality.

      02/16/2022

        Excellent product, very satisfied.

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