Recombinant Human NOTCH1 protein, His-tagged

• Cat.No.: NOTCH1-385H
• Product Overview: Recombinant Human NOTCH1 Protein (Ala19-Gln526), fused to His-tag at the C- terminus, was produced by HEK293 Cells expression system.

Specification

• Description: NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair regeneration of skeletal muscles.NOTCH1 is a prevalent signaling pathway in T cell acute lymphoblastic leukemia (T-ALL).The NOTCH signaling pathway
  is a conserved signaling cascade that regulates many aspects of development and homeostasis in multiple organ systems.The proto-oncogene NOTCH1 is frequently mutated in around 1% of patients with chronic lymphocytic leukemia (CLL). NOTCH1 mutations in oral squamous cell carcinoma (OSCC) frequently occur near the ligand-binding region. These mutations change the domain structure of this protein and affect the ligand binding activity. When NOTCH1 is activated by ligand binding, NOTCH1 intracellular domain (NICD) is cleaved from the cell membrane
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Ala19
• Form: Liquid in sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human NOTCH1 consists of 518 amino acids and predicts a molecular mass of 55 kDa.
• Protein length: Ala19-Gln526
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.5 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Reconstitution: NULL
• Publication:
  The Special Role of JAK/STAT, and Notch Signaling Pathways in Cancer Pathogenesis (2023)

Gene Information

• Gene Name: NOTCH1
• Official Symbol: NOTCH1
• Synonyms: hN1; AOS5; TAN1; AOVD1
• Gene ID: 4851
• mRNA Refseq: NM_017617.5
• Protein Refseq: NP_060087.3
• MIM: 190198
• UniProt ID: P46531

Reviews

01/24/2021

Continuous access to up-to-date training materials ensured that our team remained well-versed in the product's capabilities.

05/09/2017

The product's evolution in response to user feedback showcased a dedication to meeting the evolving needs of researchers.

11/04/2016

The product's compatibility with open science practices positively influenced our decision to adopt it for our experiments.

Q&As

How does NOTCH1 contribute to the regulation of embryonic development and tissue homeostasis?

NOTCH1 contributes to the regulation of embryonic development and tissue homeostasis by influencing cell differentiation and proliferation.

What are the ligands or interacting partners of NOTCH1, and how do they regulate its activation?

The ligands or interacting partners of NOTCH1 include Delta-like 1 (DLL1), Delta-like 3 (DLL3), Delta-like 4 (DLL4), Jagged 1, and Jagged 2, regulating its activation.

What is the primary function of Neurogenic locus notch homolog protein 1 (NOTCH1) in cellular processes and signaling?

Neurogenic locus notch homolog protein 1 (NOTCH1) functions in cellular processes, primarily playing a role in the Notch signaling pathway.

What diseases or conditions are associated with dysregulation or abnormal expression of NOTCH1?

Dysregulation or abnormal expression of NOTCH1 is associated with conditions such as cancer, cardiovascular diseases, and developmental disorders.

In which cellular compartments is NOTCH1 predominantly localized?

NOTCH1 is predominantly localized on the cell membrane, where it undergoes proteolytic cleavage upon activation.

Are there therapeutic implications or potential targets related to NOTCH1 in diseases involving Notch signaling?

There are potential therapeutic implications related to NOTCH1 in diseases involving Notch signaling, making it a target for drug development.

How does NOTCH1 participate in the Notch signaling pathway, and what is its role in cell fate determination?

NOTCH1 participates in the Notch signaling pathway by interacting with ligands such as Delta and Jagged, influencing cell fate determination.