Active Recombinant Human PDGFB Protein, His-tagged

• Cat.No.: PDGFB-515H
• Product Overview: Recombinant Human PDGFB (NP_148937.1) (Ser82-Thr190) was expressed with a polyhistidine tag at the N-terminus.

Specification

• Source: Yeast
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, 25 % Glycerol, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human PDGFB at 10μg/mL (100μL/well) can bind human PDGFRb-Fch, the EC50 of human PDGFRb-Fch is 0.05-0.2 μg/mL.
• Molecular Mass: The recombinant human PDGFB consists of 124 amino acids and predicts a molecular mass of 14.3 kDa.
• Protein length: Ser82-Thr190
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PDGFB platelet-derived growth factor beta polypeptide [ Homo sapiens ]
• Official Symbol: PDGFB
• Synonyms: PDGFB; platelet-derived growth factor beta polypeptide; platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) , SIS; platelet-derived growth factor subunit B; becaplermin; oncogene SIS; SSV; PDGF-2; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; PDGF2; c-sis; FLJ12858
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• MIM: 190040
• UniProt ID: P01127