Recombinant Human PIK3CB/PIK3R1, His-tagged, Active

• Cat.No.: PIK3CB/PIK3R1-391H
• Product Overview: Recombinant human full-length p110beta and human p85alpha were co-expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag on both proteins. MW=111 kDa (p110β), ~86 kDa (p85α).

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110β catalytic subunit (PIK3CB) plays a role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. In animal model of prostate tumor formation induced by the tumor suppressor PTEN loss, ablation of p110β impedes tumorigenesis with a concomitant diminution of AKT phosphorylation.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PIK3CB phosphoinositide-3-kinase, catalytic, beta polypeptide [ Homo sapiens ],PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens ]
• Synonyms: PIK3CB; phosphoinositide-3-kinase, catalytic, beta polypeptide; PI3K; PIK3C1; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237; PtdIns-3-kinase p110; PI3-kinase p110 subunit beta; Phosphatidylinositol-4, 5-bisphosphate 3-kinase; catalytic subunit beta isoform; EC 2.7.1.153; PI3-kinase p110 subunit beta; PtdIns-3-kinase p110; PI3Kbeta; PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); p85; GRB1; p85-ALPHA
• Gene ID: 5291,5295
• mRNA Refseq: NM_006219,NM_181504
• Protein Refseq: NP_006210,NP_852556
• UniProt ID: P42338, P27986
• Chromosome Location: 3q21, 5q13.1
• MIM: 602925,171833
• Pathway: Acute myeloid leukemia; Apoptosis; B cell receptor signaling pathway; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; Inositol phosphate metabolism; Insulin signaling pathway; Jak-STAT signaling pathway; Leukocyte transendothelial migration; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Phosphatidylinositol signaling; Progesterone-mediated oocyte maturation; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Type II diabetes mellitus; VEGF signaling pathway; mTOR signaling pathway
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; inositol or phosphatidylinositol kinase activity; insulin receptor substrate binding; nucleotide binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; transferase activity; ErbB-3 class receptor binding