Recombinant Human RRM1, GST-tagged

• Cat.No.: RRM1-2444H
• Product Overview: Recombinant Human RRM1 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 593-792
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: RRM1 ribonucleotide reductase M1 [ Homo sapiens ]
• Official Symbol: RRM1
• Synonyms: RRM1; ribonucleotide reductase M1; ribonucleotide reductase M1 polypeptide; ribonucleoside-diphosphate reductase large subunit; ribonucleotide reductase, R1 subunit; ribonucleotide reductase, large subunit; ribonucleoside-diphosphate reductase subunit M1; R1; RR1; RIR1
• Gene ID: 6240
• mRNA Refseq: NM_001033
• Protein Refseq: NP_001024
• MIM: 180410
• UniProt ID: P23921
• Chromosome Location: 11p15.5
• Pathway: E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem
• Function: ATP binding; oxidoreductase activity; purine nucleotide binding; ribonucleoside-diphosphate reductase activity; ribonucleoside-diphosphate reductase activity

Citation

Cell-cycle-dependent phosphorylation of RRM1 ensures efficient DNA replication and regulates cancer vulnerability to ATR inhibition.

Journal: Oncogene    PubMed ID: 32712628    Data: 2021/1/7

Authors: Zhen Shu, Zhen Li, Guo Chen

Article Snippet:Briefly, intracellular RNR activity was measured using the cell lysates from H1299 cells expressing WT, S559A, or S559D RRM1.Briefly, intracellular RNR activity was measured using the cell lysates from H1299 cells expressing WT, S559A, or S559D RRM1.. In vitro RNR activity was measured using purified GST tagged RRM1 proteins mixed with equal volume of recombinant His6-RRM2 protein (100 ng) (RRM2-29474TH, Creative BioMart Inc.). . The cell lysates and reconstructive RNR were incubated with reaction buffer containing 50 mM Hepes buffer pH 7.2,10 mM DTT, 20 μM FeCl3, 5 mM magnesium acetate, 50 μM CDP, 300 μM C14-CDP, and 2 mM ATP.The cell lysates and reconstructive RNR were incubated with reaction buffer containing 50 mM Hepes buffer pH 7.2,10 mM DTT, 20 μM FeCl3, 5 mM magnesium acetate, 50 μM CDP, 300 μM C14-CDP, and 2 mM ATP.