Recombinant Human RRM2, His-tagged

• Cat.No.: RRM2-29474TH
• Product Overview: Recombinant full-length Human RRM2 with a N terminal His-tag, 409 amino aicds with tag, predicted MWt 47kDa inclusive of tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 389 amino acids
• Description: This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X.
• Conjugation: HIS
• Molecular Weight: 47.000kDa inclusive of tags
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, pH 8
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MLSLRVPLAP ITDPQQLQLS PLKGLSLVDK ENTPPALSGT RVLASKTARR IFQEPTEPKT KAAAPGVEDEPLLRENPRRF VIFPIEYHDI WQMYKKAEAS FWTAEEVDLS KDIQHWESLK PEERYFISHV LAFFAASDGI VNENLVERFS QEVQITEARC FYGFQIAMEN IHSEMYSLLI DTYIKDPKER EFLFNAIETM PCVKKKADWA LRWIGDKEAT YGERVVAFAA VEGIFFSGSF ASIFWLKKRGLMPGLTFSNE LISRDEGLHC DFACLMFKHL VHKPSEERVR EIIINAVRIE QEFLTEALPV KLIGMNCTLM KQYIEFVADR LMLELGFSKV FRVENPFDFM ENISLEGKTN FFEKRVGEYQ RMGVMSSPTE NSFTLDADF
• Sequence Similarities: Belongs to the ribonucleoside diphosphate reductase small chain family.

Gene Information

• Gene Name: RRM2 ribonucleotide reductase M2 [ Homo sapiens ]
• Official Symbol: RRM2
• Synonyms: RRM2; ribonucleotide reductase M2; ribonucleotide reductase M2 polypeptide; ribonucleoside-diphosphate reductase subunit M2
• Gene ID: 6241
• mRNA Refseq: NM_001034
• Protein Refseq: NP_001025
• MIM: 180390
• Uniprot ID: P31350
• Chromosome Location: 2p25-p24
• Pathway: Cell Cycle, Mitotic, organism-specific biosystem; E2F mediated regulation of DNA replication, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; G1/S Transition, organism-specific biosystem
• Function: oxidoreductase activity; ribonucleoside-diphosphate reductase activity; ribonucleoside-diphosphate reductase activity; transition metal ion binding

Citation

Cell-cycle-dependent phosphorylation of RRM1 ensures efficient DNA replication and regulates cancer vulnerability to ATR inhibition.

Journal: Oncogene    PubMed ID: 32712628    Data: 2021/1/7

Authors: Zhen Shu, Zhen Li, Guo Chen

Article Snippet:Briefly, intracellular RNR activity was measured using the cell lysates from H1299 cells expressing WT, S559A, or S559D RRM1.Briefly, intracellular RNR activity was measured using the cell lysates from H1299 cells expressing WT, S559A, or S559D RRM1.. In vitro RNR activity was measured using purified GST tagged RRM1 proteins mixed with equal volume of recombinant His6-RRM2 protein (100 ng) (RRM2-29474TH, Creative BioMart Inc.). . The cell lysates and reconstructive RNR were incubated with reaction buffer containing 50 mM Hepes buffer pH 7.2,10 mM DTT, 20 μM FeCl3, 5 mM magnesium acetate, 50 μM CDP, 300 μM C14-CDP, and 2 mM ATP.The cell lysates and reconstructive RNR were incubated with reaction buffer containing 50 mM Hepes buffer pH 7.2,10 mM DTT, 20 μM FeCl3, 5 mM magnesium acetate, 50 μM CDP, 300 μM C14-CDP, and 2 mM ATP.