Recombinant Human S100A8 & S100A9 Heterodimer Protein, His & Flag tagged

• Cat.No.: S100A8 & S100A9-2217H
• Product Overview: Recombinant Human S100A8 (NP_002955.2) (Met1-Glu93), fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Human S100A9 (NP_002956.1) (Met1-Pro114), fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the S100A8 & S100A9 heterodimer was purified.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&Flag
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human S100A8 & S100A9 heterodimer comprises 103 amino acids (S100A8) and 122 amino acids (S100A9). It has a calculated molecular mass of 12.2 kDa (S100A8) and 14.2 kDa (S100A9).
• Protein length: Met1-Glu93&Met1-Pro114
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

02/01/2022

Dependable protein quantification, ensures experimental accuracy.

10/29/2020

Consistent western blot results, a lab essential.

03/12/2020

Expert protein engineering support, essential for our projects.

Q&As

How do alterations in S100A8 expression impact cellular stress responses and aging?

Changes in S100A8 expression impact cellular stress responses, with implications for aging and age-related diseases.

How does S100A8 contribute to the pathogenesis of cancer, especially in terms of tumor growth and metastasis?

S100A8 contributes to cancer development, influencing tumor cell proliferation and metastatic potential.

What is the impact of S100A8 on wound healing and tissue repair mechanisms?

S100A8 is involved in wound healing, affecting tissue repair and regeneration.

How does S100A8 interact with calcium ions and what are the implications for inflammatory processes?

S100A8 binds calcium ions, modulating inflammatory processes and cytokine production.

How does S100A8 influence the development and progression of cardiovascular diseases?

S100A8 affects cardiovascular disease development, impacting inflammation and vascular integrity.

What role does S100A8 play in the immune response, particularly in autoimmune diseases?

S100A8 plays a significant role in the immune response, impacting the pathogenesis of autoimmune diseases.

What are the effects of S100A8 on skin homeostasis and the development of skin disorders?

S100A8 influences skin health, playing a role in maintaining skin barrier function and in skin disorders.