Recombinant Human SIRT1 Protein, GST-tagged

• Cat.No.: SIRT1-113H
• Product Overview: Recombinant N-terminal GST-tagged human SIRT1 protein (193-747) was expressed in E. coli.

Specification

• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: GST
• Molecular Mass: 89.2 kDa
• Protein length: 193-747
• Purity: ≥70% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 pmole of 7-amino-4-methylcoumarin per minute at 25 centigrade in 50 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, containing 125 μM p53 peptide amino acids 379-382 (Arg-His-Lys-Lys (e-acetyl)-AMC), and 3 mM NAD+.
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM sodium phosphate, pH 7.2, with 100 mM sodium chloride and 20% glycerol

Gene Information

• Gene Name: SIRT1 sirtuin 1 [ Homo sapiens (human) ]
• Official Symbol: SIRT1
• Synonyms: SIRT1; sirtuin 1; SIR2; SIR2L1; SIR2alpha; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacylase sirtuin-1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; sirtuin type 1; EC 2.3.1.286
• Gene ID: 23411
• mRNA Refseq: NM_012238
• Protein Refseq: NP_036370
• MIM: 604479
• UniProt ID: Q96EB6