| Description : |
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
86.2 kDa |
| AA Sequence : |
MSNPGGRRNGPVKLRLTVLCAKNLV KKDFFRLPDPFAKVVVDGSGQCHST DTVKNTLDPKWNQHYDLYIGKSDSV TISVWNHKKIHKKQGAGFLGCVRLL SNAINRLKDTGYQRLDLCKLGPNDN DTVRGQIVVSLQSRDRIGTGGQVVD CSRLFDNDLPDGWEERRTASGRIQY LNHITRTTQWERPTRPASEYSSPGR PLSCFVDENTPISGTNGATCGQSSD PRLAERRVRSQRHRNYMSRTHLHTP PDLPEGYEQRTTQQGQVYFLHTQTG VSTWHDPRVPRDLSNINCEELGPLP PGWEIRNTATGRVYFVDHNNRTTQF TDPRLSANLHLVLNRQNQLKDQQQQ QVVSLCPDDTECLTVPRYKRDLVQK LKILRQELSQQQPQAGHCRIEVSRE EIFEESYRQVMKMRPKDLWKRLMIK FRGEEGLDYGGVAREWLYLLSHEML NPYYGLFQYSRDDIYTLQINPDSAV NPEHLSYFHFVGRIMGMAVFHGHYI DGGFTLPFYKQLLGKSITLDDMELV DPDLHNSLVWILENDITGVLDHTFC VEHNAYGEIIQHELKPNGKSIPVNE ENKKEYVRLYVNWRFLRGIEAQFLA LQKGFNEVIPQHLLKTFDEKELELI ICGLGKIDVNDWKVNTRLKHCTPDS NIVKWFWKAVEFFDEERRARLLQFV TGSSRVPLQGFKALQGAAGPRLFTI HQIDACTNNLPKAHTCFNRIDIPPY ESYEKLYEKLLTAIEETCGFAVETR TRPLEQKLISEEDLAANDILDYKDD DDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
